VEMS: A TOOL TO QUANTIFY iTRAQ LABELED SAMPLES

Introduction: The goal of many MS-based proteomics experiments nowadays is to quantify changes in the abundance of the proteomes across several samples of biological interest. The iTRAQ labeling method is a powerful relative quantitation technique that combined with liquid chromatography coupled to tandem mass spectrometry allows quantify up to eight different samples simultaneously. The transformation of the multiple spectra containing different protein expression values is a challenging task. We have developed an integrated tool for database dependent interpretation, quantitation and database storage for iTRAQ labeled samples able to handle various input data formats from instruments from different manufacturers. Users can download the Web Server from http://personal.cic.biogune.es/rmatthiesen/.